Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2432166 | Fish & Shellfish Immunology | 2012 | 7 Pages |
Interferon gamma (IFN-γ) is a cytokine that plays a very important role in defining Th1 immune response in all vertebrates. In this study, recombinant IFN-γ (rIFN-γ) from the olive flounder (Paralichthys olivaceus) was produced in an Escherichia coli system using a pET expression vector. Stimulation of whole kidney leukocytes (immune-related cells) in vitro with the resulting rIFN-γ significantly induced the gene expression of interleukin-1β (IL-1β), signal transducer and activator of transcription 1 (STAT1), CXCL13-like chemokine (CXCL13), and IFN-γ. rIFN-γ also weakly induced the expression of IL-1β, tumor necrosis factor-α (TNF-α), CXCL13, and IFN-γ in olive flounder-derived HINAE (non-immune) cells. The effects of rIFN-γ against Edwardsiella tarda infection in vivo were assessed by intraperitoneally injecting a mixture of rIFN-γ (100 ng) and E. tarda (1 × 105 CFU/ml) into the olive flounder. The survival rate in the rIFN-γ-injected group was 60% compared to 0% in the group treated with E. tarda only, demonstrating that olive flounder IFN-γ is effective in reinforcing immune responses and preventing against edwardsiellosis.
► Recombinant interferon gamma (rIFN-γ) was produced using pET system. ► rIFN-γ can induce IL-1β, STAT1, CXCL13, and IFN-γ in kidney leukocytes. ► rIFN-γ can weakly induce IL-1β, TNF-α, CXCL13, and IFN-γ in HINAE cells. ► rIFN-γ can protect fish challenged with Edwardsiella tarda.