| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2432226 | Fish & Shellfish Immunology | 2011 | 7 Pages |
Members of the DnaJ/Hsp40 family play an important role in protein homeostasis by regulating the activity of DnaK/Hsp70. In this study, we examined the activity and function of the DnaJ from Edwardsiella tarda, a serious fish pathogen that can also infect humans and birds. In silico analysis indicated that E. tarda DnaJ contains structural features, i.e. the J domain, the glycine/phenylalanine-rich region, and the zinc-finger domain, that are conserved among Type I Hsp40. Purified recombinant DnaJ was able to stimulate the ATPase activity of DnaK. Pull-down assay indicated that DnaJ could interact specifically with DnaK. Mutation of the conserved HPD site in the J domain completely abolished the DnaK-stimulating effect of DnaJ. To examine the functional importance of DnaJ, a dnaJ-defective mutant was constructed. Compared to the wild type, the dnaJ mutant (i) was retarded in growth and more sensitive to H2O2-induced oxidative damage, (ii) dramatically reduced in general bacterial virulence and in blood dissemination capacity, and (iii) significantly weakened in the ability to block macrophage activation and to survive within macrophages. Furthermore, when used as a subunit vaccine, purified recombinant DnaJ induced protective immunity in Japanese flounder (Paralichthys olivaceus). Taken together, these results indicate that DnaJ plays an important role in the pathogenesis of E. tarda probably by functioning as a DnaK partner and that DnaJ, with its immunoprotective property, may be useful in the control of E. tarda infection in aquaculture.
► DnaJ of Edwardsiella tarda contains structural features conserved among Type I Hsp40. ► Recombinant DnaJ interacts with and stimulates the ATPase activity of DnaK. ► Mutation of the conserved HPD site abolishes the DnaK-stimulating effect of DnaJ. ► dnaJ Mutation affects H2O2 resistance, virulence, and survival in macrophages. ► Recombinant DnaJ induces protective immunity in Japanese flounder.
