| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2432424 | Fish & Shellfish Immunology | 2011 | 7 Pages |
Chemokines are a large family of chemotactic cytokines playing crucial roles in the innate immune response. In the present study, we report the cloning of a CXC chemokine gene resembling the closely related CXCL9/CXCL10/CXCL11 from the miiuy croaker Miichthys miiuy (MimiCXC). Both 5′-RACE and 3′-RACE were carried out in order to obtain the complete cDNA, which consists of a 73 bp 5′-UTR, a 369 bp open reading frame encoding 122 amino acids and a 715 bp 3′-UTR. The deduced MimiCXC contains a 19-aa signal peptide and a 103-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known CXC chemokines. It shares 4.8%–65.6% sequence identities to mammalian CXC chemokines and the highest sequence identity of 65.6% is between MimiCXC and CXCL10 chemokine. Three exons and two introns were identified in MimiCXC gene. The MimiCXC gene was constitutively expressed in all tissues tested, although at different levels. Upon induction with Vibrio anguillarum, MimiCXC gene expression was up-regulated in kidney and spleen, however, down-regulated in liver. These results indicate that MimiCXC may be involved in immune responses as well as homeostatic processes in miiuy croaker.
► A CXC chemokine gene (MimiCXC) was cloned from Miichthys miiuy. ► MimiCXC resembles the closely related CXCL9/CXCL10/CXCL11. ► MimiCXC was highly expressed in intestine. ► MimiCXC expression in kidney and spleen was up-regulated by Vibrio anguillarum.
