| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2432486 | Fish & Shellfish Immunology | 2011 | 7 Pages |
Snakehead Channa striata is an important freshwater food fish in many Southeast Asian countries. Three monoclonal antibodies (C9, C10 and D10) were developed against purified serum immunoglobulins of Channa striata (Cs-Ig) and characterized. C9 and D10 MAbs were specific to heavy chain, while C10 MAb detected only unreduced Cs-Ig in western blotting. In competitive ELISA, C9 and C10 MAbs were specific to C. striata Ig and showed no cross reactivity with serum Ig of other fish species i.e. Channa punctatus, Channa marulius, Clarias batrachus and Labeo rohita. D10 MAb showed reactivity to serum Ig of C. striata and C. marulius. In FACS analysis of gated lymphocytes, the percentage of Ig+ cells detected by C9 MAb was 18.2%, 27.7% and 10.3% in blood, spleen and kidney, respectively (n = 3, body weight 500–600 g). However, only a few cells (0.5%) were found to be Ig+ in thymus (n = 5). C9 MAb was also successfully employed to demonstrate Ig+ cells in blood smears and formalin fixed sections of spleen and kidney. These findings suggest that the spleen plays an important role in humoral immunity as compared to head kidney. Further, these MAbs can be useful immunological tool in monitoring health status of cultured C. striata.
Research highlights► Three monoclonal antibodies (MAbs) were produced against serum immunoglobulins of Channa striata. ► Two MAbs (C9 and C10) were specific to C. striata Ig. ► One MAb (D10) showed reactivity to serum Ig of C. striata and C. marulius. ► C9 MAb was employed to detect and quantify Ig+ cells in lymphoid organs and blood.
