| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2433660 | Fish & Shellfish Immunology | 2006 | 5 Pages |
Abstract
A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes.
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Authors
Arjen Companjen, Bas Heinhuis, Kelly Aspers, Jan Rombout,