Article ID Journal Published Year Pages File Type
4557773 Journal of Invertebrate Pathology 2013 5 Pages PDF
Abstract

•We observed Nocardia crassostreae in a new host Mytilus galloprovincialis.•We also document N. crassostreae infection in natural banks of flat oyster O. edulis.•We describe this detection for the first time in the Mediterranean sea.•We assess the identity of Nocardia thought sequence, phylogenetic analyses and ISH.

In this work M. galloprovincialis and O. edulis specimens were surveyed for a pathological study in the Gulf of Naples (Mediterranean sea, Campania Region, southern Italy). Clusters of Nocardia sp.-like cells were observed in histological slides. PCR amplification, sequencing and in situ hybridization were carried out in order to corroborate Nocardia species identification for both hosts. Blast results showed a 99% of maximum identity with Nocardia crassostreae sequences in Genbank. This is the first report of N. crassostreae in the new host M. galloprovincialis and, in a new area, the Mediterranean Sea.

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