Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4558219 | Journal of Invertebrate Pathology | 2010 | 4 Pages |
Abstract
Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a â¼120Â kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots.
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Authors
Erich Y.T. Nakasu, Alexandre A.P. Firmino, Simoni C. Dias, Thales L. Rocha, Hudson B. Ramos, Gustavo R. Oliveira, Wagner Lucena, Célia R. Carlini, Maria Fátima Grossi-de-Sá,