Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4558480 | Journal of Invertebrate Pathology | 2009 | 4 Pages |
Abstract
We present the first record in Spanish Mediterranean waters of the protozoan parasite Perkinsus olseni infecting the clam Ruditapes decussatus. Perkinsus infection was detected all year around albeit at a low level of infection intensity. Histological analysis, induction of zoospores and in situ hybridisation assay confirmed the presence of Perkinsus sp. The identity of the parasite was determined by species-specific PCR assay in DNA samples obtained from infected clams. Sequencing of amplified fragments showed 100% identity to the ITS region of P. olseni. We confirmed for the first time the presence of P. olseni in Spanish Mediterranean waters.
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Authors
Laurence M. Elandaloussi, Noèlia Carrasco, Ana Roque, Karl Andree, M. Dolores Furones,