Article ID Journal Published Year Pages File Type
6389552 Journal of Invertebrate Pathology 2014 4 Pages PDF
Abstract

•A SYBR Green real-time PCR suitable for the detection of S. eriocheiris from P. clarkii was developed.•It was proven to be specific, sensitive, reproducible and mensurable.•A tissue-specific pathogen infection characteristic was demonstrated.•Proliferation characteristics of S. eriocheiris in crayfish were studied.

Spiroplasma eriocheiris disease control based on sensitive quantitative methods has become a priority. A SYBR Green real-time PCR that can simultaneously detect and quantify S. eriocheiris in the freshwater crayfish Procambarus clarkii was produced and evaluated. In the asymptomatic crayfish, hemolymph exhibited the statistically greatest number of S. eriocheiris copies indicating a tissue-specific pathogen infection characteristic. The curve of the pathogen amount change in vivo assumed a very similar shape with the typical one-step growth curve. A turning point from chronic infection to acute infection was suggested from 3 to 4 days when the S. eriocheiris copies in hemolymph increased substantially.

Graphical abstractDownload full-size image

Related Topics
Life Sciences Agricultural and Biological Sciences Ecology, Evolution, Behavior and Systematics
Authors
, , , , , , , ,