| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6389592 | Journal of Invertebrate Pathology | 2013 | 4 Pages |
â¢IHC and RT-PCR was used to determine WSSV replication in naturally infected Dendronereis spp.â¢WSSV-immunoreactive nuclei present in the gut epithelium of Dendronereis spp.â¢Nested RT-PCR produced a band at 364 bp. Both results suggested that WSSV replicates in Dendronereis spp.
This study investigated whether WSSV replicates in naturally infected Dendronereis spp., a common polychaete (Nereididae) species in shrimp ponds in Indonesia. To detect WSSV replication, (i) immunohistochemistry (IHC) using a monoclonal antibody against WSSV VP28 protein and (ii) nested RT-PCR using specific primers set for the vp28 gene to detect WSSV-specific mRNA were applied. WSSV immunoreactive-nuclei were detected in the gut epithelium of the polychaete and WSSV mRNA was detected with nested RT-PCR. This, together with the IHC results, confirmed that WSSV could replicate in Dendronereis spp. This is the first report showing that WSSV replicated in a naturally infected non-crustacean host.
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