Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6389813 | Journal of Invertebrate Pathology | 2011 | 5 Pages |
We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with â¼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.
Graphical abstractqPCR expression profile of different immune related genes in Fenneropenaeus indicus after the Vibrio harveyi challenge. Download full-size image.Highlights⺠In this study, using SSH we identified 38 genes and eight hypothetical proteins in V. harveyi challenged F. indicus postlarvae. ⺠Real time gene expression analyses showed rise in expression levels of all seven different immune related transcripts in the postlarvae. ⺠Ferritn, translationally controlled tumor protein and hemocyanin were the major upregulated genes in V. harveyi challenged postlarvae.