Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8498639 | Fish & Shellfish Immunology | 2018 | 8 Pages |
Abstract
Gel-enhanced liquid chromatography coupled with tandem mass spectrometry (GeLC-MS/MS) was used to analyze the proteome of Macrobrachium rosenbergii hepatopancreas responded to Poly (I:C). GeLC-MS/MS analysis identified 515 differentially-expressed proteins with â¥1.5 and ⤠â0.5 log2 fold change. Of these, 195 differentially-expressed proteins were significantly matched to known proteins in the database, of which 102 proteins were up-regulated and 93 proteins were down-regulated. These proteins were classified into 21 categories, i.e. metabolic process, oxidative stress response, signaling, transcription, translation, cell cycle, transport, etc. Several immune factors were up-regulated upon Poly (I:C) injection. Protein-protein interaction network analysis of these immune factors identified three major protein clusters including RNAi, stress responses, and Toll pathway-proPO system, implying that Poly (I:C) activates immune responses in prawn through several mechanisms.
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Authors
Pheng Saray, Sittiruk Roytrakul, Tanapat Pangeson, Amnat Phetrungnapha,