Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8498801 | Fish & Shellfish Immunology | 2018 | 39 Pages |
Abstract
The full-length cDNA of sb-isg15 gene, encoding a predicted protein of 155 amino acids, was identified and seen to share the same characteristics as other fish and mammalian isg15 genes. Here we report the clear induction of sb-isg15 transcript levels in SAF-1â¯cells and AGs stimulated with toll-like receptor (TLR) ligands, such as polyinosinic:polycytidylic acid (poly I:C) or genomic DNA from Vibrio anguillarum (VaDNA), respectively. Furthermore, VHSV and SJNNV inoculation induced a significant degree of sb-isg15 transcription in SAF-1â¯cells and AGs. However, the relative levels of viral RNA transcription showed that SJNNV replication seems to be more efficient than VHSV in both in vitro systems. Interestingly, sb-isg15 transcript induction elicited by VaDNA was reduced in VHSV- and SJNNV-inoculated AGs, suggesting an interference prompted by the viruses against the type I IFN system. Taken together, these findings support the use of seabream AGs as a valuable experimental system to study virus-host interactions, in which sb-isg15 seems to play an important role.
Keywords
FCSISG15MACSPKRVHSVIHNVTCID50NNVL15TLRENAViral haemorrhagic septicaemia virusUblAGSribosomal protein S18LPSEpithelioma papulosum cypriniFBSEPCCPEdouble-stranded RNA-activated protein kinaseRps18PAMPsInfectious haematopoietic necrosis virus50% tissue culture infective doseIFNsISGsMOIROSinterferonsEuropean Nucleotide Archiveanalysis of varianceANOVAfoetal bovine serumfoetal calf serumcytoplasmic polyadenylation elementPhagocytesAntiviral activitylipopolysaccharideTeleostmagnetic-activated cell sortingUTR یا untranslated regions Neighbour-joiningnervous necrosis viruspathogen associated molecular patternsPropidium iodidePoly I:CPolyinosinic:polycytidylic acidmultiplicity of infectionIFN-stimulated genesHead kidneyacidophilic granulocytesReactive oxygen speciesToll-like receptors
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Authors
Daniel Álvarez-Torres, Victoria Gómez-Abellán, Marta Arizcun, Esther GarcÃa-Rosado, Julia Béjar, MarÃa P. Sepulcre,