Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8498870 | Fish & Shellfish Immunology | 2018 | 8 Pages |
Abstract
A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.
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Authors
Mirella Vazzana, Monica Celi, Marco Chiaramonte, Luigi Inguglia, Debora Russo, Vincenzo Ferrantelli, Dominique Battaglia, Vincenzo Arizza,