Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8499127 | Fish & Shellfish Immunology | 2016 | 8 Pages |
Abstract
The yellow catfish, Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. However, genomic research and resources on this species are largely unavailable and still in infancy. In the present study, we constructed a cDNA library following poly I:C injection to screen for immune response genes in the spleens of P. fulvidraco using suppression subtractive hybridization (SSH). A total of 420 putative expressed sequence tag (EST) clones were identified at 24Â h post-injection, which contain 103 genes consisting of 25 immune response genes, 12 cytoskeleton genes, 7Â cell cycle and apoptosis genes, 7 respiration and energy metabolism genes, 7 transport genes, 26 metabolism genes, 10 stress response genes, 9 translational regulation genes, and 71 unknown genes. Real-time quantitative reverse transcription-PCR (qRT-PCR) results revealed that a set of randomly selected immune response genes were identified to be up-regulated after 24Â h of poly I:C stimulation compared to controls. Our study provides an annotation of immune genes in detail and insight into fish immunity.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Aquatic Science
Authors
Qiu-Ning Liu, Zhao-Zhe Xin, Xin-Yue Chai, Sen-Hao Jiang, Chao-Feng Li, Dai-Zhen Zhang, Chun-Lin Zhou, Bo-Ping Tang,