Development of PCR primers specific for the amplification and direct sequencing of gyrB genes from microbacteria, order Actinomycetales Fulltext Access 9 Pages 2005
Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari Fulltext Access 9 Pages 2005
Fluorescence in situ hybridisation coupled to ultra small immunogold detection to identify prokaryotic cells using transmission and scanning electron microscopy Fulltext Access 9 Pages 2005
Evaluation of a loop-mediated isothermal amplification method for rapid detection of channel catfish Ictalurus punctatus important bacterial pathogen Edwardsiella ictaluri Fulltext Access 9 Pages 2005
Development of a quantitative real-time polymerase chain reaction targeted to the toxR for detection of Vibrio vulnificus Fulltext Access 9 Pages 2005
Development of a rapid colorimetric time-kill assay for determining the in vitro activity of ceftazidime and tobramycin in combination against Pseudomonas aeruginosa Fulltext Access 9 Pages 2005
Immunomagnetic separation of Toxoplasma gondii oocysts using a monoclonal antibody directed against the oocyst wall Fulltext Access 9 Pages 2005
Development of a strain-specific assay for detection of viable Lactobacillus sp. HOFG1 after application to cattle feed Fulltext Access 9 Pages 2005
Allele-specific PCR method based on rfbS sequence for distinguishing Salmonella gallinarum from Salmonella pullorum: serotype-specific rfbS sequence polymorphism Fulltext Access 9 Pages 2005
Compatibility of the green fluorescent protein and a general nucleic acid stain for quantitative description of a Pseudomonas putida biofilm Fulltext Access 9 Pages 2005
Measurement of bacterial adhesion-in vitro evaluation of different methods Fulltext Access 9 Pages 2005
Reverse engineering of biochemical equations from time-course data by means of genetic programming Fulltext Access 10 Pages 2005
PCR-DGGE analysis for the identification of microbial populations from Argentinean dry fermented sausages Fulltext Access 10 Pages 2005
Changes of viability and composition of the Escherichia coli flora in faecal samples during long time storage Fulltext Access 10 Pages 2005
Probing leukocyte traffic in lymph from oro-nasal mucosae by cervical catheterization in a sheep model Fulltext Access 10 Pages 2005
Screening microarrays of novel monoclonal antibodies for binding to T-, B- and myeloid leukaemia cells Fulltext Access 10 Pages 2005
Analysis of synonymous codon usage in H5N1 virus and other influenza A viruses Fulltext Access 10 Pages 2005
The origin of the genetic code: theories and their relationships, a review Fulltext Access 10 Pages 2005
Increased precision of microbial RNA quantification using NASBA with an internal control Fulltext Access 10 Pages 2005
Identification of conserved regulatory RNA structures in prokaryotic metabolic pathway genes Fulltext Access 10 Pages 2005
Designing a neural network for the constraint optimization of the fitness functions devised based on the load minimization of the genetic code Fulltext Access 10 Pages 2005
Thermosynthesis as energy source for the RNA World: A model for the bioenergetics of the origin of life Fulltext Access 10 Pages 2005
“Bioplutonism” and the evolutionary implications of beneficial genes from another biosphere Fulltext Access 10 Pages 2005
Multidrug transporter MexB of Pseudomonas aeruginosa: overexpression, purification, and initial structural characterization Fulltext Access 10 Pages 2005
Cloning and characterization of the rat HIF-1α prolyl-4-hydroxylase-1 gene Fulltext Access 10 Pages 2005
Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli Fulltext Access 10 Pages 2005
Tight transcriptional regulation of foreign genes in insect cells using an ecdysone receptor-based inducible system Fulltext Access 10 Pages 2005
Development of a versatile cassette for directional genome walking using cassette ligation-mediated PCR and its application in the cloning of complete lipolytic genes from Bacillus species Fulltext Access 10 Pages 2005
Green fluorescent protein for detection of the probiotic microorganism Escherichia coli strain Nissle 1917 (EcN) in vivo Fulltext Access 10 Pages 2005
Automated and unbiased classification of chemical profiles from fungi using high performance liquid chromatography Fulltext Access 10 Pages 2005
In vivo microdialysis sampling of cytokines produced in mice given bacterial lipopolysaccharide Fulltext Access 10 Pages 2005
An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water Fulltext Access 10 Pages 2005
Feasibility of assessment of regulatory lipids in breath condensate as potential presymptomatic harbingers of pulmonary pathobiology Fulltext Access 10 Pages 2005
Rapid identification of dairy lactic acid bacteria by M13-generated, RAPD-PCR fingerprint databases Fulltext Access 10 Pages 2005
Lytic enzyme, labiase for a broad range of Gram-positive bacteria and its application to analyze functional DNA/RNA Fulltext Access 10 Pages 2005
The use of RAPD genomic fingerprinting to study relatedness in strains of Acidithiobacillus ferrooxidans Fulltext Access 10 Pages 2005
Specific detection of cytopathogenic Listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis Fulltext Access 10 Pages 2005
Non-disruptive, real-time analyses of the metabolic status and viability of Streptococcus mutans cells in response to antimicrobial treatments Fulltext Access 10 Pages 2005
Effect of natamycin on the enumeration, genetic structure and composition of bacterial community isolated from soils and soybean rhizosphere Fulltext Access 10 Pages 2005
Stable genetic transformation of the ectomycorrhizal fungus Pisolithus tinctorius Fulltext Access 10 Pages 2005
Development of real-time PCR using Minor Groove Binding probe to monitor the biological control agent Candida oleophila (strain O) Fulltext Access 10 Pages 2005
Capillary electrophoresis single-strand conformation polymorphism analysis for monitoring soil bacteria Fulltext Access 10 Pages 2005
Association between blood plasma urea nitrogen levels and reproductive fluid urea nitrogen and ammonia concentrations in early lactation dairy cows Fulltext Access 10 Pages 2005
IgG subclass-independent improvement of antibody-dependent cellular cytotoxicity by fucose removal from Asn297-linked oligosaccharides Fulltext Access 10 Pages 2005
A new method for adjusting neural response jitter in the STRF obtained by spike-trigger averaging Fulltext Access 10 Pages 2005
Retino-cortical information transmission achievable with a retina implant Fulltext Access 10 Pages 2005
Effects of prepartum lipid supplementation on FSH superstimulation and transferable embryo recovery in multiparous beef cows Fulltext Access 10 Pages 2005
Fertility of cryopreserved sperm in three bulls with different Robertsonian translocations Fulltext Access 10 Pages 2005
Timing of ovulation in relation to onset of estrus and LH peak in yak (Poephagus grunniens L.) Fulltext Access 10 Pages 2005
Conventional slow freezing, vitrification and open pulled straw (OPS) vitrification of rabbit embryos Fulltext Access 10 Pages 2005
Sandwich type ELISA and a fluorescent cytometric microbead assay for quantitative determination of hepatitis B virus X antigen level in human sera Fulltext Access 10 Pages 2005
Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle Fulltext Access 10 Pages 2005
Effect of dietary protein on embryo recovery rate and quality in superovulated heifers Fulltext Access 10 Pages 2005
Lactation weight loss influences subsequent reproductive performance of sows Fulltext Access 10 Pages 2005
Fertility in the mare after repeated transvaginal ultrasound-guided aspirations Fulltext Access 10 Pages 2005
Prevalence of aerobic and anaerobic uterine bacteria during peripartum period in normal and dystocia-affected buffaloes Fulltext Access 10 Pages 2005
Capacitation, acrosome function and chromatin structure in stallion sperm Fulltext Access 10 Pages 2005
Effect of eCG dose and ovulation induction treatments on embryo recovery and in vitro development post-vitrification in two selected lines of rabbit does Fulltext Access 10 Pages 2005
Effect of feeding level on progesterone concentration in early pregnant multiparous sows Fulltext Access 10 Pages 2005
Effects of in vitro fertilization conditions on preimplantation development and quality of pig embryos Fulltext Access 10 Pages 2005
Validation of a 13,14-dihydro-15-keto-PGF2α enzymeimmunoassay and its application for reproductive health monitoring in postpartum buffaloes Fulltext Access 10 Pages 2005
The use of trimeric isoleucine-zipper fusion proteins to study surface-receptor-ligand interactions in natural killer cells Fulltext Access 10 Pages 2005
Epitope analysis of PPARγ monoclonal antibody Pγ48.34A and its application for screening PPARγ ligands Fulltext Access 10 Pages 2005
Detection of HIV-1-specific CTL responses in Clade B infection with Clade C Peptides and not Clade B consensus peptides Fulltext Access 10 Pages 2005
Granulocyte contamination dramatically inhibits spot formation in AIDS virus-specific ELISpot assays: Analysis and strategies to ameliorate Fulltext Access 10 Pages 2005
Analysis of the binding of monoclonal and polyclonal antibodies to the glycoproteins of antigenic variants of human respiratory syncytial virus by surface plasmon resonance Fulltext Access 10 Pages 2005
Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system Fulltext Access 10 Pages 2005
Use of a new immunoassay to measure PrPSc levels in scrapie-infected sheep brains reveals PrP genotype-specific differences Fulltext Access 10 Pages 2005
Establishment of a simple and quantitative immunospot assay for detecting anti-type II collagen antibody using an infrared fluorescence imaging system (IFIS) Fulltext Access 10 Pages 2005
Human tumor cell lysates as a protein source for the detection of cancer antigen-specific humoral immunity Fulltext Access 10 Pages 2005
Cryopreserved dendritic cells for intratumoral immunotherapy do not require re-culture prior to human vaccination Fulltext Access 10 Pages 2005
A multiplexed and miniaturized serological tuberculosis assay identifies antigens that discriminate maximally between TB and non-TB sera Fulltext Access 10 Pages 2005
Fertility and hatchability of eggs laid in the pullet-to-breeder transition period and in the initial production period Fulltext Access 10 Pages 2005
Design and selection of an intrabody library produced de-novo for the non-structural protein NSP5 of rotavirus Fulltext Access 10 Pages 2005
Assays for the assessment of neutralizing antibody activities against Severe Acute Respiratory Syndrome (SARS) associated coronavirus (SCV) Fulltext Access 10 Pages 2005
Identification and characterization of antibodies that bind GPIIb/IIIa: Antagonist complexes Fulltext Access 10 Pages 2005
Detection of circulating autoantibodies directed against the asialoglycoprotein receptor using recombinant receptor subunit H1 Fulltext Access 10 Pages 2005
Affinity ranking of antibodies using flow cytometry: Application in antibody phage display-based target discovery Fulltext Access 10 Pages 2005
In vitro modification of solid phase multiple antigenic peptides/autoantigens with 4-hydroxy-2-nonenal (HNE) provide ideal substrates for detection of anti-HNE antibodies and peptide antioxidants Fulltext Access 10 Pages 2005
A comparative study of immunomagnetic methods used for separation of human natural killer cells from peripheral blood Fulltext Access 10 Pages 2005
Detection of T cell memory to measles virus in experimentally infected rhesus macaques by cytokine flow cytometry Fulltext Access 10 Pages 2005
Efficient gene transfer into normal human B lymphocytes with the chimeric adenoviral vector AD5/F35 Fulltext Access 10 Pages 2005
Evaluation of the concept of heterology in a monoclonal antibody-based ELISA utilizing direct hapten linkage to polystyrene microtiter plates Fulltext Access 10 Pages 2005