Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10107704 | International Journal of Food Microbiology | 2005 | 10 Pages |
Abstract
The new primers PF1 and PF8 were chosen from the sequence of gene dps, a putative glucan synthase gene, found across all the ropy P. damnosus strains isolated, from both wine or cider, and also in a ropy O. oeni strain. To our knowledge, this is the first time that an EPS-producing O. oeni strain is described. Glucan biosynthesis was assessed by agglutination tests done with Streptococcus pneumoniae type 37-specific antibodies, which specifically detect glucan-producing cells. The results show that there is a direct correlation between glucan production and detection of gene dps. Therefore, Dps is considered a candidate for the glucan synthase enzyme responsible for EPS production by ropy strains of P. damnosus and O. oeni.
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Authors
Emilie Walling, Emmanuel Gindreau, Aline Lonvaud-Funel,