Fractionation of polysaccharides by gradient non-solvent precipitation: A review

This paper provides comprehensive and detailed directions for how to fractionate a new polysaccharide using this method. It is suggested to select a suitable non-solvent and establish the fractionation procedure according to the curve of the polysaccharide recovery as a function of the non-solvent concentration. Fractionation by gradient non-solvent precipitation is based on the difference in solubility of polysaccharides. Therefore, fractionation may be affected by the precipitation conditions and those factors that can affect the solubility of polysaccharides. During fractionation, those factors that negatively affect fractionation should be strictly controlled.