Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10143206 | Meat Science | 2019 | 11 Pages |
Abstract
Intramuscular fat (IMF) plays an important role in pork quality. However, differences in the adipogenic regulation of IMF content between pig longissimus thoracis (LT) and semitendinosus (ST) remain unclear. Here, we found that IMF content of 180-day-old pig LT was greater than that of pig ST. Furthermore, lipid accumulation was earlier and greater in LT intramuscular preadipocytes (L-IMA) than in ST intramuscular preadipocytes (S-IMA) during differentiation. Interestingly, glucose consumption was lower in L-IMA than in S-IMA. Moreover, monounsaturated fatty acid content was greater in L-IMA than in S-IMA, whereas polyunsaturated fatty acid content was lower. Levels of the expression of key adipogenic genes were higher in L-IMA than S-IMA. Compared with S-IMA, adipogenic signals were more activated in L-IMA after adipogenic induction. In conclusion, IMF deposition differences between pig LT and ST were due to different glucose consumption, fatty acid composition, expression of key adipogenic genes and level of activating adipogenic signals between S-IMA and L-IMA during adipogenesis.
Keywords
TAGSREBF1SNAP23PPARGMDILEPRsemitendinosusGLUT4Longissimus thoracis et lumborumstearoyl-CoA desaturaseFABP4MUFALTLSterol regulatory element-binding transcription factor 1ERKSCDGAPDHmTORLongissimus thoracisAdipogenesisAktfatty acid synthasePolyunsaturated fatty acidmonounsaturated fatty acidPUFAImatriacylglyceroldifferentiation mediumPigIMFFasnIntramuscular fat contentGlucose transporter type 4mammalian target of rapamycinhaematoxylin-eosinfatty acid binding protein 4protein kinase BExtracellular regulated protein kinaseIntramuscular fatglyceraldehyde-3-phosphate dehydrogenaseLeptin receptor
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Authors
Fen-Fen Chen, Ying-Qian Wang, Guo-Rong Tang, Shu-Ge Liu, Rui Cai, Yun Gao, Yun-Mei Sun, Gong-She Yang, Wei-Jun Pang,