Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10972334 | International Dairy Journal | 2011 | 6 Pages |
Abstract
Vitamin B12 and folate deficiencies in poor Southern African communities lead to mandatory fortification of basic foodstuffs. Since folate- and B12-enriched kefir offers a cheaper alternative, regimes to include Propionibacterium freudenreichii (PAB) strains into kefir grains (KG) were investigated: two levels of PAB cell concentrations; freeze-drying KG to preserve PAB activity; and repeated PAB culture additions. Elevated B12 and folate levels and PCR results confirmed inclusion of PAB in all KG. Repeated inoculations with freeze-dried cultures (PAB concentration 1 Ã 108 cfu mLâ1) delivered the highest B12 and folate production rate and concentration after 3 d. The best treatment (freeze-dried inoculum (5 Ã 107 PAB cfu mLâ1) reacted once, followed by freeze-drying) delivered 186% Recommended Dietary Allowance (RDA) (B12) and 19% RDA (folate) per 200 mL serving. Freeze-drying preserved PAB activity in KG.
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Authors
Jessy Van Wyk, R. Corli Witthuhn, Trevor J. Britz,