The free and total myo-inositol contents of early lactation and seasonal bovine milk

A high performance anion-exchange chromatographic method employing pulsed amperometric detection was applied to the determination of endogenous free and total myo-inositol in bovine milk, for which there is limited information. The contents and trend variability of myo-inositol in milk from extensively pasture-fed cows during early lactation and across a production season were therefore evaluated. Free and total myo-inositol in seasonal milk were within the ranges of 2.3–4.5 mg 100 g−1 and 5.3–8.7 mg 100 g−1, respectively. This novel information will both improve understanding of the expression of innate myo-inositol in bovine milk, and provide manufacturers with information that can enhance formulation capability related to the production of cow's milk-based products