Determination of total potentially available nucleosides in bovine, caprine, and ovine milk

The total potentially available nucleosides (TPAN) in bovine, caprine, and ovine milk were analyzed through the sequential application of phosphatase, pyrophosphatase, and nuclease enzyme treatments prior to high performance liquid chromatographic analysis of released nucleosides. The contributions to TPAN from polymeric nucleotides, monomeric nucleotides, and nucleotide adducts were then calculated. Ovine milk contained the highest concentration of TPAN, i.e., 374.1 μmol dL−1, with lower concentrations in caprine milk (97.4 μmol dL−1) and bovine milk (7.9 μmol dL−1). Ovine milk contained the highest concentrations of each of the different nucleoside and nucleotide forms, and bovine milk contained the lowest.