| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2450252 | Meat Science | 2012 | 4 Pages |
Activities of 19 hydrolases were estimated in cell suspensions of 40 Brochothrix thermosphacta strains, isolated from meat and meat products packaged under various conditions, by using API ZYM® test. These strains produced: acidic phosphatase, esterase C4, esterase/lipase C8, α-chymotrypsin, leucine arylamidase, β-glucosidase and α-glucosidase. The predominating biotype produced only the first four of the listed enzymes. The extracellular lipolytic activity for bromo-chloro-indolyl-caprylate was detected only in culture broth supernatants of 4 strains thereby indicating that esterase/lipase C8 is cell-bound. 13 of the strains displayed proteolytic activity for albumin at 4 °C (0.31–2.07 U) while 9 strains showed this activity at 25 °C (0.11–1.21 U). Only 4 strains digested albumin at both temperatures. Thus the meat spoilage potential of B. thermosphacta strains results not only from digestion of carbohydrates but also from their proteolytic activity.
► Brochothrix thermosphacta show proteolytic activity in 4 and 25 °C. ► The proteolytic activity is independent of bacterial origin. ► Brochothrix thermosphacta show extracellular lipolytic activity only in 25 °C. ► B. thermosphacta lipases preferring esters of glycerol and short chain fatty acids.
