Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2451875 | Meat Science | 2007 | 7 Pages |
Abstract
Polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was applied to the specific identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus). The use of a common reverse primer, together with forward specific primers for red deer, fallow deer, and roe deer, allowed the selective amplification of the desired cervid sequences. The specificity of each primer pair was verified by PCR analysis of DNA from various game and domestic meats. The assay can be useful for the accurate identification of meats from cervid species, avoiding mislabeling or fraudulent species substitution in meat products.
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Authors
V. Fajardo, I. González, I. López-Calleja, I. Martín, M. Rojas, P.E. Hernández, T. García, Rosario Martín,