| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2586439 | Food and Chemical Toxicology | 2009 | 5 Pages |
To evaluate the fusaproliferin (FUS) production, Fusariumsubglutinans ITEM 2404 was grown in a liquid medium of potato being this mycotoxin purified by high-performance liquid chromatography (HPLC) with a C18 semipreparative column using a mobile phase of acetonitrile/H2O using gradient conditions. The purity of the fusaproliferin was verified by analytical HPLC, ultraviolet absorbance measurements, LC/MS–MS, 1H NMR spectroscopy. The isolated FUS was shown to be free of impurities and can be used as a standard for routine analysis. The pure fusaproliferin was utilized to study the biological activity on Escherichiacoli and Staphylococcusaureus. This study demostred that FUS not showed significant antimicrobial activity against these microorganisms.
