Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2587104 | Food and Chemical Toxicology | 2008 | 8 Pages |
Abstract
Schizandrae chinensis, a traditional Chinese medicine herb, has been used to treat hepatitis B disease in Chinese hospital clinic. We have isolated two bioactive compounds, deoxyschizandrin and γ-schizandrin, from S. chinensis. In the present, we reported that deoxyschizandrin and γ-schizandrin could induce apoptosis in human promyelocytic leukemia cells (HL-60), as characterized by DNA fragmentation and poly (ADP) ribose polymerase (PARP) cleavage. Further molecular analysis showed that deoxyschizandrin and γ-schizandrin caused the loss of mitochondrial membrane potential (ÎΨm), cytochrome c release from mitochondrion to cytosol, truncation of Bid protein, and activation of caspase-3 and -9. However, they did not increase the intracellular level of reactive oxygen species (ROS). Antioxidants such as N-acetyl cysteine (NAC) and catalase did not block the apoptosis induced by deoxyschizandrin or γ-schizandrin. These findings suggest that deoxyschizandrin and γ-schizandrin-induced apoptosis in HL-60 cells involved ROS-independent mitochondrial dysfunction pathway.
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Authors
Shigang Lin, Makoto Fujii, De-Xing Hou,