Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4362866 | Food Microbiology | 2014 | 6 Pages |
Abstract
Most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. The aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. The list of targets include, among the others, Listeria spp., Salmonella spp., Shigella spp., Escherichia coli spp., Campylobacter spp., Clostridium spp. and Staphylococcus aureus. The accuracy of detection was determined by using ATCC strains as positive and negative controls. The achieved sensitivity of each of assays was 1 pg of genomic DNA, which was equivalent to â¼1 cfu. The working ranges of the TaqMan® Real-time PCR assays, when used quantitatively on cheese and meat samples inoculated with serial dilution of Listeria spp., Listeria monocytogenes, S. aureus, Salmonella enterica, Shigella boydii, E. coli O157:H7, Bacillus cereus, Campylobacter coli, Yersinia enterocolitica, Enterobacter sakazakii and Pseudomonas aeruginosa was 108 cfu/g to 104 cfu/g. No matrix interferences were observed.
Keywords
Related Topics
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Agricultural and Biological Sciences
Food Science
Authors
Paola Cremonesi, Laura Francesca Pisani, Cristina Lecchi, Fabrizio Ceciliani, Pieranna Martino, Armand Sanchez Bonastre, Avo Karus, Claudia Balzaretti, Bianca Castiglioni,