Article ID Journal Published Year Pages File Type
4362874 Food Microbiology 2014 6 Pages PDF
Abstract

•An impedance system was used to measure microbial growth in real foods.•The detection level was similar to optical density methods in laboratory liquid media.•A good correlation was observed between inoculum levels and times to detection.•Growth rates estimations were sufficiently accurate for modeling in a few cases.•Impedance possible alternative method to study microbial growth in real food.

The suitability of indirect impedance to accurately measure microbial growth in real food matrices was investigated. A variety of semi-solid and liquid food products were inoculated with Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Lactobacillus plantarum, Pseudomonas aeruginosa, Escherichia coli, Salmonella enteriditis, Candida tropicalis or Zygosaccharomyces rouxii and CO2 production was monitored using a conductimetric (Don Whitely R.A.B.I.T.) system. The majority (80%) of food and microbe combinations produced a detectable growth signal. The linearity of conductance responses in selected food products was investigated and a good correlation (R2 ≥ 0.84) was observed between inoculum levels and times to detection. Specific growth rate estimations from the data were sufficiently accurate for predictive modeling in some cases. This initial evaluation of the suitability of indirect impedance to generate microbial growth data in complex food matrices indicates significant potential for the technology as an alternative to plating methods.

Related Topics
Life Sciences Agricultural and Biological Sciences Food Science
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