| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4366343 | International Journal of Food Microbiology | 2016 | 4 Pages |
•Modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by supplementation with cefotetan and cefoxitin.•The ability of the modified medium to isolate Campylobacter from chicken carcass rinse was compared to that of normal mCCDA.•Improved mCCDA yielded significantly better isolation rate and selectivity than those achieved on using normal mCCDA.
Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is increasing. Here we evaluated the potential of cephamycins for use as supplements to improve modified charcoal-cefoperazone-deoxycholate agar (mCCDA) by replacing cefoperazone with the same concentrations (32 mg/L) of cefotetan (modified charcoal-cefotetan-deoxycholate agar, mCCtDA) and cefoxitin (modified charcoal-cefoxitin-deoxycholate agar, mCCxDA). In chicken carcass rinse samples, the number of mCCDA plates detecting for Campylobacter (18/70, 26%) was significantly lower than that of mCCtDA (42/70, 60%) or mCCxDA plates (40/70, 57%). The number of mCCDA plates (70/70, 100%) that were contaminated with non-Campylobacter species was significantly higher than that of mCCtDA (20/70, 29%) or mCCxDA plates (21/70, 30%). The most common competing species identified using mCCDA was ESBL-producing E. coli, while Pseudomonas species frequently appeared on mCCtDA and mCCxDA.
