| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4366617 | International Journal of Food Microbiology | 2015 | 8 Pages |
•Identification of original Propionibacterium sources in dairy farms•Demonstration of genetic diversity of autochthonous Propionibacterium in dairy farms•RAPD, PFGE and MLST to assess the genetic diversity of Propionibacterium•Analysis of molecular profiles from Brazilian Propionibacterium with reference strains
Dairy propionibacteria are used as ripening cultures for the production of Swiss-type cheeses, and some strains have potential for use as probiotics. This study investigated the biodiversity of wild dairy Propionibacteria isolates in dairy farms that produce Swiss-type cheeses in Minas Gerais State, Brazil. RAPD and PFGE were used for molecular typing of strains and MLST was applied for phylogenetic analysis of strains of Propionibacterium freudenreichii. The results showed considerable genetic diversity of the wild dairy propionibacteria, since three of the main species were observed to be randomly distributed among the samples collected from different farms in different biotopes (raw milk, sillage, soil and pasture). Isolates from different farms showed distinct genetic profiles, suggesting that each location represented a specific niche. Furthermore, the STs identified for the strains of P. freudenreichii by MLST were not related to any specific origin. The environment of dairy farms and milk production proved to be a reservoir for Propionibacterium strains, which are important for future use as possible starter cultures or probiotics, as well as in the study of prevention of cheese defects.
