Typing of Lactobacillus sanfranciscensis isolates from traditional sourdoughs by combining conventional and multiplex RAPD–PCR profiles

In the present work, a rapid and reproducible molecular method, based on the combination of conventional and multiplex RAPD–PCR reactions, was developed for typing Lactobacillus sanfranciscensis isolates from traditional sourdoughs. At first, four random primers, two used singly and two combined with the primer RD1, were chosen on the basis of their differentiating capability and reproducibility. The four resulting profiles for each isolate were integrated into a unique profile to be statistically treated by cluster analysis. The method was validated on 58 L. sanfranciscensis isolates coming from three traditional Italian sourdoughs. This new RAPD method was useful for determining the genomic diversity within the L. sanfranciscensis species. In particular, the intraspecific diversity of this species seemed to be related to the sourdough origin.

► Development of a RAPD–PCR method for typing L. sanfranciscensis isolates. ► Combination of RAPD profiles from conventional and multiplex RAPD–PCR reactions. ► Method validation on L. sanfranciscensis from three traditional Italian sourdoughs. ► Intraspecific biodiversity of L. sanfranciscensis related to the sourdough origin.