Article ID Journal Published Year Pages File Type
4367836 International Journal of Food Microbiology 2012 5 Pages PDF
Abstract

We performed a comprehensive safety assessment of a probiotic based on the whole genome sequence and corresponding phenotypes. This was performed on Lactobacillus plantarum JDM1, a widely used commercial probiotic strain in China. The minimal inhibitory concentrations (MICs) of sixteen antibiotics and the biogenic amine production of JDM1 were tested to supplement a traditional oral toxicity test. In total, fifty-one antibiotic resistance-associated genes, one hundred twenty-six virulence-associated genes, and twenty-three adverse metabolism-associated genes were found in JDM1. However, there were no toxin or hemolysin encoding genes, and safety-associated genes were rarely transferable. This approach can be generalized to provide a deep safety investigation of novel probiotic strains and greatly reveal the potential danger determinants and their molecular mechanisms. However, this kind of analysis reveals the theoretical maximum risk level as not all genes are efficient depending on environmental conditions.

► Comprehensive safety evaluation process of probiotics based on genome and phenotypes. ► The safety of L. plantarum JDM1 was validated by this combined approach. ► Complete genome facilitates study of safety and stability for each probiotics.

Related Topics
Life Sciences Agricultural and Biological Sciences Food Science
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