Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4368251 | International Journal of Food Microbiology | 2009 | 9 Pages |
Abstract
Two Streptococcus thermophilus phages (ALQ13.2 and ÏAbc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525Â bp in length for the pac-type phage ALQ13.2 and 34,882Â bp for the cos-type phage ÏAbc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and ÏAbc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the ÏAbc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDS-PAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of ÏAbc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.
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Authors
Daniela M. Guglielmotti, Hélène Deveau, Ana G. Binetti, Jorge A. Reinheimer, Sylvain Moineau, Andrea Quiberoni,