Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4369536 | International Journal of Food Microbiology | 2008 | 6 Pages |
Abstract
A total of 225 samples from poultry farms and the surrounding environment were screened for vancomycin-resistant enterococci (VRE) and bifunctional aminoglycoside-resistant enterococci using conventional microbiological tests and a nanoplex polymerase chain reaction (PCR) assay. Three (1.3%) of the samples were found to contain vancomycin-resistant isolates (MIC > 256 µg/mL) that had a vanA genotype. The three vanA positive VRE isolates were identified as different species. Only one isolate (Enterococcus faecium F 4/13_54) was sensitive to teicoplanin (MIC < 0. 12-0.35 µg/mL); the other two VRE (E. faecalis A 21_35 and E. gallinarum F 5/10_1) were resistant to teicoplanin (MIC 3.6 â 16 µg/mL). The vanC genotype was observed in nine (4%) of the samples collected. High-level gentamicin-resistant (HLGR) enterococci (with MIC ranging between 100 and 500 µg/mL) were detected in 44 samples. However, only 40 of these were found to possess the aac(6â²)-aph(2â³) gene. The overall prevalence of VRE among the samples from the poultry farms and environment was 5.3%, but the prevalence of the clinically significant vanA VRE was 1.3%, and the prevalence of bifunctional aminoglycoside-resistant enterococci was slightly higher, at 19.5%.
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Authors
Yean Yean Chan, Mohd Hafiz B. Abd Nasir, Mohd Azli B. Yahaya, Noor Mohamad Amin B. Salleh, Azril Deenor B. Md Dan, Abd Majid B. Musa, M. Ravichandran,