Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4559111 | Food Control | 2016 | 11 Pages |
•Salmonella detection from naturally contaminated poultry in 12–24 h using biosensors.•Fiber optic and light scattering sensor together provides high sensitivity and accuracy.•Label-free light scattering sensor screens Salmonella from food products.•Comprehensive and integrated application of biosensor and immuno/molecular assays.•First report that integrates both fiber optic and laser sensor for pathogen detection.
Salmonella-related foodborne infections present a major public health problem worldwide despite more stringent regulations. Salmonella enterica serovars Enteritidis and Typhimurium are the two most frequent causes of poultry related outbreaks; therefore, their rapid and accurate detection would improve Salmonella control at the farm, processing plant, and at retail. In this study, we investigated if a fiber optic immunosensor and light scattering sensor, BARDOT (bacterial rapid detection using optical scattering technology) could facilitate the detection of these two serovars in naturally contaminated poultry (n = 50). The fiber optic sensor with a detection limit of 103 CFU/ml identified S. enterica in selective enrichment broth in less than 12 h. The colonies (1.0 ± 0.2 mm) produced by plating the enriched samples on selective XLT4 agar for 13–15 h were scanned using BARDOT and S. enterica was identified after matching individual colony scatter patterns to the scatter image library with a sample-to-answer time of about 24 h. Both sensors identified 4 positive samples (8%), which corresponded to the results of the USDA-FSIS protocol, PCR, and lateral flow immunoassays. The colony scatter patterns identified all natural isolates as S. Enteritidis, which was further verified by serovar-specific PCR. The sensors used individually or in combination demonstrate potential for accurate and rapid detection of S. enterica in poultry.