Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4559898 | Food Control | 2010 | 7 Pages |
Abstract
Application of real-time PCR and SYBR Green for selective enumeration of probiotic bacteria in a lyophilised probiotic product sold as an OTC drug, containing Lactobacillus gasseri, Bifidobacterium infantis and Enterococcus faecium was evaluated. Maxwell DNA Purification Kit was found the most efficient and repeatable. Among nine pairs of primers tested, the following were selected: Enc-F, Enc-R for E. faecium, Lgas-3/Lgas-2 for L. gasseri and BiINF-1/BiINF-2 for B. infantis. Comparison of plate counts and real-time PCR analysis of 10 batches of product showed that on average 62% of lactobacilli, 45% of enterococci and 2% of bifidobacteria preserved their culturability.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Food Science
Authors
Bojana Bogovič Matijašić, Tanja Obermajer, Irena Rogelj,