| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4561894 | Food Research International | 2012 | 9 Pages |
Myctophids (Benthosema pterotum) were regarded as low-valued small deep-sea fishes. In this study, we however identified that B. pterotum contains high levels of proteins, free amino acids and some functional components (e.g. phospholipids, polyunsaturated fatty acids and minerals). Converting Myctophids from a low-valued fish to a high value-added protein source was thus attempted. The content of nitrogen compounds in the protein hydrolysates of B. pterotum (BPH) (by autolysis) accounted for 53.6% of peptides with Mw less than 1500 Da. BPH was also identified to possess high levels of reducing power (1.26 ± 0.04 abs), Fe2 +-chelating activity (95.73 ± 2.05%) and DPPH scavenging activity (94.01 ± 1.00%), which correspond to 100 ppm of ascorbic acid, 100 ppm of EDTA and 20 ppm of ascorbic acid, respectively. We further determined that the in vitro effective concentrations of BPH for eliminating 50% free radicals (EC50) on DPPH scavenging, Fe2 +-chelating, reducing power and superoxide oxide dismutase (SOD) activity are 5.66 μM, 3.74 μM, 12.27 μM and 56.37 μM, respectively. For food safety, BPH was evaluated by both Ames test (using Salmonella Typhimurium TA98) and acute toxicity in a rat model. No mutagenicity arose on the levels of BPH ranging from 625 to 5000 μg with/without metabolic activation. No treatment-related changes (e.g. general condition, body weight, ophthalmology, hematology, and histopathology) were found in rats. For the no-observed-adverse-effect-level (NOAEL), BPH was estimated to be no less than 15 g/kg in both male and female rats. The autolytic hydrolysates of BPH were concluded to be safe. Accordingly, BPH is an ideal raw material for the developments of protein-rich anti-oxidative healthy products.
► B. pterotum were proved containing copious of biomasses and attractive advantages. ► Hydrolysats of B. pterotum (BPH) contain abundant nutrients and activities. ► BHP is also free of toxic wax esters and poisonous trace elements. ► BHP is an ideal protein-rich source to develop into healthy products.
