Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4561962 | Food Research International | 2008 | 7 Pages |
Abstract
A barley β-glucan concentrate prepared according to a novel technology was further purified and subjected to detailed structural characterization by NMR spectroscopy. β-Glucan was hydrolysed with β-glucan-4-glucanohydrolase (lichenase). Fractions of hydrolysate were collected using an HPLC-fraction collector. Intact β-glucan and the major fractions collected were subjected to MALDI-TOF–MS and NMR analyses. The two major oligosaccharides produced by lichenase hydrolysis of purified barley β-glucan were identified as β-d-Glc p-(1 → 4)- β-d-Glc p-(1 → 3)-β-d-Glc p and β-d-Glc p-(1 → 4)-β-d-Glc p-(1 → 4)-β-d-Glc p-(1 → 3)-β-d-Glc p based on 13C and 1H NMR data. Spectrums were similar to those documented for barley β-glucan in the literature.
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Authors
B.S. Ghotra, T. Vasanthan, F. Temelli,