| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4564052 | LWT - Food Science and Technology | 2011 | 11 Pages |
The rheological characterizations of African yam bean (Sphenostylis stenocarpa) protein dispersions were investigated. Isoelectrically precipitated protein-IPalk and IPsalt isolates obtained from alkaline and salt extractions respectively were more soluble than calcium precipitated proteins (CaPalk and CaPsalt) at pH 3, 7 and 8. Regression analysis showed that Power law, Casson and Bingham rheological models adequately described rheological behaviors of S. stenocarpa protein dispersion. However, Power law gave the best fit. The flow behavior indices (n), at different ionic strength, pH, and temperature media were less than unity, indicating that S. stenocarpa protein dispersion exhibited pseudoplastic behaviors under the conditions tested. Salt extracted proteins were more pseudoplastic than alkali extracted counterpart with n for salt extracted proteins (IPsalt & CaPsalt) lower than that of alkali extracted protein (CaPalk & CaPsalt). This is a numerical indication that salt extracted S. stenocarpa proteins were of larger shear-thinning tendency than the alkali extracted proteins. The consistency coefficients, k of isoelectrically precipitated protein (0.305–0.327 Pasn) were significantly (P < 0.05) higher than that of calcium proteinates in the range ranged 0.167–0.180 Pasn. Both isoelectrically precipitated proteins and calcium proteinates exhibited yield stress, however, isoelectrically precipitated S. stenocarpa protein exhibited significantly (P < 0.05) higher yield stress (0.275–0.308 Pa) than the calcium proteinates (0.148–0.165 Pa). The effect of temperature on apparent viscosity of the proteins was evaluated using an Arrhenius-type equation. The activation energies (Ea) obtained were in the range 33–51.2 and 42.6–55.5 Jmol−1 for calcium proteinate and isoelectrically precipitated protein respectively.
