| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 542813 | Microelectronic Engineering | 2014 | 4 Pages |
•Effectively concentrating bacteria by MNP based immunoassay using microfluidics.•Microchannels are fabricated easily and reproducibly in one step.•Different concentrations of Escherichia coli were detected with high degree of repeatability.•Inexpensive yet powerful tool to quantify bacteria is demonstrated.
Here we report development of a magnetic nanoparticles based immunosensor chip comprising of specially designed circular microchannel and embedded miniature permanent magnet. The present process of fabrication allows a rapid and reliable tool to create microchannels with ease and at a low cost. The chip permits easy in-flow capture of bacteria, their visual detection and quantification as demonstrated by Escherichia coli detection. Such a technique enhances capture efficiency and makes quantification of captured antigens simple.
Graphical abstractOne step, easy process was employed to fabricate circular micro-capillary with permanent magnet embedded in PDMS. The chip was tested successfully as biosensor for the detection of bacteria using magnetic nanoparticles.Figure optionsDownload full-size imageDownload as PowerPoint slide
