| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 544399 | Microelectronic Engineering | 2012 | 4 Pages |
An optically transparent micro cell chip consisting of a polydimethylsiloxane (PDMS) channel structure and cover glass was fabricated to examine the intracellular calcium responses of a single MG-63 cell (human osteoblast-like bone cell) to shear stress with various magnitudes. To supply stable mechanical stimulation to cells seeded on the micro channel structure, a computer-controlled pneumatic system was used to generate pressure-driven fluid flow in the micro channel. Its effectiveness was verified by comparing the measured flow rate of the working fluid induced by the syringe pump and the lab-designed pneumatic system. Intracellular calcium responses in cells were measured using a laser-scanning microscope under varying extracellular mechanical environments. Based on the temporal responses of the intracellular calcium expression, the cells had multiple peaks with a 154 ± 11 s average period. This period was maintained with respect to shear stress of various magnitudes from 0.61 to 2.86 Pa.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We fabricate an optically transparent micro cell chip. ► We use a lab-designed control system to supply stable shear stress to cells. ► We measure the temporal responses of the intracellular calcium expression. ► MG-63 Cells had multiple peaks to shear stress with various magnitudes.
