| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 544402 | Microelectronic Engineering | 2012 | 4 Pages |
Direct and fast (10s of seconds) deposition of flame-made, high surface-area aerosol films on polymers and polymeric microfluidic devices is demonstrated. Uniform TiO2 nanoparticle films were deposited on cooled poly(methyl methacrylate) (PMMA) substrates by combustion of titanium(IV) isopropoxide (TTIP) – xylene solution sprays. Films were mechanically stabilized by in situ annealing with a xylene spray flame. Plasma-etched microfluidic chromatography columns, comprising parallel microchannels were also coated with such nanoparticle films without any microchannel deformation. These microcolumns were successfully used in metaloxide affinity chromatography (MOAC) to selectively trap phosphopeptides on these high surface-area nanostructured films. The chips had a high capacity retaining 1.2 μg of standard phosphopeptide. A new extremely fast method is developed for MOAC microchip stationary phase fabrication with applications in proteomics.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Rapid flame aerosol deposition and in situ annealing of nano-TiO2 on temperature sensitive polymers. ► Deposition in polymeric (PMMA) microfluidics without channel deformation. ► On-chip phosphopeptide enrichment/separation by metaloxide affinity chromatography for proteomics.
