Article ID Journal Published Year Pages File Type
5541220 International Dairy Journal 2017 25 Pages PDF
Abstract
The investigation of the authenticity and classification of milk powder is particularly important. The use of time-resolved fluorescence could help develop a new method to assist milk powder classification and assess composition. This study used static and time-resolved fluorescence to differentiate powder milk samples. We observed fluorescence lifetimes of (in ns) 1.5 ± 0.2, 3.5 ± 0.2 and 6.0 ± 0.2 for skimmed; 1.3 ± 0.2, 3.3 ± 0.2 and 6.0 ± 0.4 for whole; and 1.4 ± 0.2, 3.4 ± 0.3 and 6.0 ± 0.4 for semi-skimmed powder milk at 270/335 nm excitation/emission. In addition, we observed fluorescence lifetimes of (in ns) 1.4 ± 0.1, 6.4 ± 0.2 and 14.8 ± 1.1 for skimmed; 1.6 ± 0.2, 6.8 ± 0.2 and 29.5 ± 1.7 for whole; and 1.7 ± 0.1, 6.8 ± 0.2 and 26.1 ± 1.3 for semi-skimmed powder milk at 315/468 nm excitation/emission. Employing analysis of variance, we differentiated all powder samples and achieved accuracy of 100% by discriminant analysis at 315/468 nm excitation/emission fluorescence. These results indicate that time-resolved fluorescence can assist in the classification of powder milk and its composition.
Related Topics
Life Sciences Agricultural and Biological Sciences Food Science
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