Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5543416 | Meat Science | 2017 | 6 Pages |
Abstract
In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 μg/kg (CLIN) and 6.8 μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra â/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC â MS/MS with a correlation coefficient > 0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles.
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Authors
Jie He, Nan Wu, Pengjie Luo, Ping Guo, Jianwen Qu, Shuying Zhang, Xin Zou, Feiyao Wu, Huijuan Xie, Can Wang, Wenxiao Jiang,