Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5560018 | Food and Chemical Toxicology | 2017 | 9 Pages |
â¢Cruciferous vegetables increased activity and protein expression of cytochrome P450 (CYP) 1A1 and CYP1A2 after 7 d intake.â¢Increased CYP1A activities were sustained after 60 d of cruciferous vegetable intake.â¢Increased protein expression of CYP1As was not sustained at 30 d and 60 d of cruciferous vegetable intake.â¢Apiaceous vegetables did not affect CYP1A1 activity or CYP1A1 and CYP1A2 protein expression.â¢Apiaceous vegetables increased CYP1A2 activity and decreased sulfotransferase 1A1 activity after 7 d of intake.
Cruciferous and apiaceous vegetables may be chemopreventive due to their ability to modulate carcinogen-metabolizing enzymes but whether the effects on such enzymes are sustained over time is unknown. To examine the short- and long-term effects of the vegetables, rats were fed one of four diets for 7, 30, or 60Â d: AIN-93G, CRU (21% cruciferous vegetables-fresh broccoli, green cabbage, watercress), API (9% apiaceous vegetables - fresh parsnips, celery), or APIÂ +Â CRU (10.5% CRUÂ +Â 4.5% API). Although CRU increased activity and protein expression of cytochrome P450 (CYP) 1A1 and CYP1A2 after 7Â d, only activity was sustained after 30 and 60Â d. There was a trend towards an interaction between the length of feeding period and CRU for CYP1A1 activity; activity increased with greater time of feeding. API increased CYP1A2 activity but decreased sulfotransferase 1A1 activity after 7Â d, although not at later times. Altogether, increased CYP1A activity by CRU was maintained with long term feeding while protein amount decreased, suggesting influence by mechanisms other than, or in addition to, transcriptional regulation. Thus, response patterns and interactions with length of feeding may differ, depending upon the types of vegetables and enzymes, requiring caution when interpreting the results of short-term feeding studies.
Graphical abstractDownload high-res image (159KB)Download full-size image