A novel approach for simultaneous determination of E/Z-fluoxastrobins in vegetables and fruits by UHPLC-DAD

•A method was shown for simultaneous determination of E/Z-fluoxastrobins.•A novel sample preparation had been introduced for pesticide residues.•The established pretreatment has advantage with other pretreatments.•The proposed method was simple, effective, easy, inexpensive and good accuracy.

In this work, a novel analytical method was established for simultaneous determination of E-fluoxastrobin and Z-fluoxastrobin in vegetables and fruits. After extracted by acetonitrile, the samples were cleansing with dispersive solid liquid extraction (d-SLE) process, and analyzed by ultra high performance liquid chromatography coupled to diode array detection (UHPLC-DAD). The effectiveness of the optimized method was validated by determining the linearity (R2 > 0.99), sensitivity (limits of quantification of 30 μg/kg), recovery (71.3-113.2%), and precision (relative standard deviations, RSDs ≤ 13.9%) in eight vegetable and fruit samples. The results suggested that this method was simple, reliable, and feasible to determine the residues of E-fluoxastrobin and Z-fluoxastrobin in vegetable and fruit samples, which could be further applied to 160 various vegetable and fruit samples.