| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5767809 | Food Research International | 2017 | 6 Pages |
â¢Nitric oxide activates glycogen synthase kinase-3.â¢Guanosine 3â²,5â²-cyclic monophosphate mediates glycogen synthase kinase-3 activation.â¢Glycogen synthase kinase-3 mediates isoflavone production.â¢Glycogen synthase kinase-3 activates chalcone synthase and isoflavone synthase.
The role of glycogen synthase kinase-3 (GSK-3) in the nitric oxide-guanosine 3â²,5â²-cyclic monophosphate (NO-cGMP)-induced isoflavone production in soybean sprouts was examined. Inhibitors and donors of NO, cGMP, and GSK-3 inhibitor were added to UV-B irradiated sprouts. Results showed that NO, with cGMP, induced the expression of GSK-3 under UV-B radiation. Protein kinase G (PKG) was shown to be involved in NO-cGMP-induced GSK-3 activation. GSK-3 elevated activity and expression levels of chalcone synthase (CHS) and isoflavone synthase (IFS), and increased isoflavone accumulation.
Graphical abstractThe NO-cGMP/PKG pathway activated GSK-3 under UV-B radiation. GSK-3 up regulated the activity and expression levels of CHS and IFS, and increased isoflavone production.Download high-res image (161KB)Download full-size image
