Hot air drying process promotes lignification of Lentinus edodes

•Hot air drying (HAD) promoted the lignification of L. edodes.•The increase of total polyphenol advanced lignification of L. edodes during HAD.•Lignification of L. edodes resulted from the elevation of POD and PAL activities.•Free water and immobilized water mobilized significantly during HAD process.

Hot air drying (HAD) is the most commonly used method for processing of postharvest Lentinus edodes in industry, which may promote lignification in the mushroom, affecting nutrition and edibility of L. edodes. The mechanism underlying this, however, remains unclear, so it's difficult to control and reduce the phenomenon. Results suggested that lignifying structure, the shrinkage of hyphae tissues and tightening of tubular structure, were observed in SEM owing to transportation of moisture distribution. The transverse relaxation time of free water and immobilized water mobilized significantly, and only 1.26 ± 0.34 and 1.15 ± 0.17 g/100 g bound water was left in the stipe and pileus after 12 h. Furthermore, the increase of lignin content illustrated that HAD process promoted lignification of L. edodes, along with the increase of total polyphenol content and cellulose content. HAD process promoted the lignification of L. edodes by increasing the content of lignin, which was attributed to the elevation of total polyphenol content and regulated by phenylalanine ammonia lyase (PAL) and peroxidases (POD). These results will serve as a theoretical underpinning for elucidating the mechanism of lignification in L. edodes and controlling this deterioration phenomenon during HAD process in food processing.