Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5849759 | Food and Chemical Toxicology | 2015 | 37 Pages |
Abstract
Systemic exposure was measured in humans after hair dyeing with oxidative hair dyes containing 2.0% (A) or 1.0% (B) [14C]-p-phenylenediamine (PPD). Hair was dyed, rinsed, dried, clipped and shaved; blood and urine samples were collected for 48 hours after application. [14C] was measured in all materials, rinsing water, hair, plasma, urine and skin strips. Plasma and urine were also analysed by HLPC/MS/MS for PPD and its metabolites (B). Total mean recovery of radioactivity was 94.30% (A) or 96.21% (B). Mean plasma Cmax values were 132.6 or 97.4 ng [14C]-PPDeq/mL, mean AUC0-â values 1415 or 966 ng [14C]-PPDeq/mL*hr in studies A or B, respectively. Urinary excretion of [14C] mainly occurred within 24 hrs after hair colouring with a total excretion of 0.72 or 0.88% of applied radioactivity in studies A or B, respectively. Only N,Nâ²-diacetylated-PPD was detected in plasma and the urine. A TK-based human safety assessment estimated margins of safety of 23.3- or 65-fold relative to respective plasma AUC or Cmax values in rats at the NOAEL of a toxicity study. Overall, hair dyes containing PPD are unlikely to pose a health risk since they are used intermittently and systemic exposure is limited to the detoxified metabolite N,Nâ²-diacetyl-PPD.
Keywords
PPDsystemic exposure doseNAT1SCCPParaphenylenediamineTDANOAELMOStmaxSCCsCmaxLLOQLSCAUCHPLC/MS/MSt1/2IARC یا International Agency for Research on CancerToxicokineticsMargin of safetyLOD یا Limit of detectionmaximal plasma concentrationHair dyesWorld Health Organisationbody mass indexBMIliquid scintillation countingConsumer exposurearea under the curveelimination rate constantelimination half-lifesedbody weightWHO
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Authors
Gerhard J. Nohynek, Julie A. Skare, Wim J.A. Meuling, Kenneth R. Wehmeyer, Albertus Th H.J. de Bie, Wouter H.J. Vaes, Eric K. Dufour, Rolf Fautz, Winfried Steiling, Mario Bramante, Herve Toutain,