Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5850251 | Food and Chemical Toxicology | 2014 | 13 Pages |
Abstract
The direct modulation of Antrodia cinnamomea (AC) on the prominent role of liver fibrosis-hepatic stellate cells (HSCs) in situ remains unclear. Firstly, the administration of A. cinnamomea mycelial extract (ACME) could improve liver morphology and histological changes including collagen formation and GPT activity in the liver of thioacetamide (TAA)-injured rats. The morphology and fatty acid restore of TAA-induced HSCs (THSCs) returned to the non-chemical induced HSCs (NHSCs) type as measured by immunofluorescence and Oil Red O staining. PPARγ was upregulated associated with the lowering of α-SMA protein in NHSC-ACME. ACME inhibited the MMP-2 activity in NHSCs by gelatin Zymography. After LC-MS/MS, the cytoskeleton (tubulin, lamin A) and heat shock protein 8 in NHSC-ACME, and guanylate kinase, brain-specific kinase, SG-II and p55 proteins were downregulated in THSC-ACME. Whereas MHC class II, SMC6 protein, and phospholipase D were upregulated in NHSC-ACME. Furthermore, PKG-1 was downregulated in NHSC-ACME and upregulated in THSC-ACME. SG-II and p55 proteins were downregulated in NHSC-ACME and THSC-ACME by Western blotting. Taken together, the beneficial effect of A. cinnamomea on the induction of HSC cellular proteins is potentially applied as an alternative and complementary medicine for the prevention and amelioration of a liver injury.
Keywords
PPAR-γp55Smc6Secretogranin IIaHSCsACMEMTSDAPIAntrodia cinnamomeaMMP-2PMSTAADMEMα-SMAFBS4′,6-diamidino-2-phenylindoleROSalpha smooth muscle actinReversionProteomic analysisThioacetamidefetal bovine serumHepatic stellate cellsMass spectrometryactivated hepatic stellate cellsphenazine methosulfatematrix metalloproteinase 2Dulbecco’s modified eagle’s mediumstructural maintenance of chromosomesliquid chromatographyPeroxisome proliferator-activated receptor gammaReactive oxygen speciestumor necrosis factor receptor 1
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Authors
Yi-Ren Chen, Kai-Ting Chang, May-Jywan Tsai, Chia-Hung Lee, Kao-Jean Huang, Henrich Cheng, Yen-Peng Ho, Jian-Chyi Chen, Hsueh-Hui Yang, Ching-Feng Weng,